The 33-year-old was performing during her ‘Piece of me’ residency when she made the comment, reports femalefirst.co.uk.
“Look how beautiful the girls are, oh my God. Men, frankly, can suck my f***ing toe. But girls, I love you,” she said.
Meanwhile, Spears, who has sons Sean Preston, 9 and Jayden James, 8, with former husband Kevin Federline, is currently single since her split from producer Charlie Ebersol earlier this year.
While the pair are believed to still be friends, it has been claimed that they broke up because he didn’t want to get married.
A source said: “Britney had been pressuring Charlie for the last several months about getting married. She is at a point where she wants to have another child, and very much wants to be married again. Charlie had been very honest about not being ready to get married.”